CG201
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CG201
Sigma
cGMP Enzyme Immunoassay Kit
sufficient for 96 assays
Description
| Application | Non-radioactive, competitive immunoassay for the quantitation of cGMP in biological fluids (serum, plasma, urine and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well. |
Properties
| usage | sufficient for 96 assays |
| shipped in | wet ice |
| storage temp. | −20°C |
Safety
| Hazard Codes | F,C |
| Risk Statements | 11-20/21/22-35 |
| Safety Statements | 3-16-26-29-36/37/39-45 |
| RIDADR | UN 3316 9/PG 2 |
Components
| Standard component | Acetic Anhydride 1×1 mL |
| Assay Buffer 2 1×30 mL | |
| cGMP-Alkaline Phosphatase Conjugate 1×5 mL | |
| cGMP Assay Layout Sheet 1×1 sheet | |
| cGMP EIA Antibody Rabbit Anti-cGMP 1×5 mL | |
| 5 Cycle Logit-Log Paper 1×1 sheet | |
| Cyclic GMP Standard 1×0.5 mL | |
| Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 each | |
| p-Nitrophenyl Phosphate Substrate Solution 1×20 mL | |
| Plate Sealer 1 each | |
| Stop Solution 1×5 mL | |
| Triethylamine 1×2 mL | |
| Wash Buffer Concentrate 1×30 mL |
References
| reference | Chard, T. An Introduction to Radioimmunoassay and Related Techniques 4th ed., Amsterdam , (1990) |
| Tijssen, P. Practice and Theory of Enzyme Immunoassays Amsterdam , (1985) |
